ABSTRACT
Objective To improve the understandazation of manifestation of lung metastases and awareness of characteristics. Methods 21 cases of lung metastases were collected by the clinical diagnosis during Jan 2005 to Dec 2008, and its manifestation of chest X -ray and CT imaging was analysed to explore the characteristics. Results The chest X - ray was a conventional method for lung metastases .CT, especially HRCT, can provide more comprehensive and accurate image information. 21 cases of lung metastases included 17 cases of hematogenic metastasis, accounting for 80% , 2 cases of lymphatic metastasis and 2 cases of direct metastasis, each accounting for 10% . Primary tumor + lung typical manifestation can help to make more precise diagnosis. Conclusion Chest X - ray and CT are an important for lung metastases in the clinical diagnosis, treatment, program settings, and efficacy of assessment.
ABSTRACT
Objective To investigate the effects of small interference RNA (siRNA) targeting vascular endothelial growth factor (VEGF) on VEGF expression and cell proliferation of cervical carcinoma HeLa cells,in order to provide a theoretical basis for the treatment of human cervical carcinoma.Methods siRNAs plasmids targeting VEGF165 were constructed and stably transfected into HeLa cells.HeLa group,Mock group and VEGF siRNA group were set up.The expressions of VEGF mRNA and protein in stable transfectants were confirmed by RT-PCR,Western blotting and ELISA assay,respectively.The cell proliferation was analyzed by MTT and flow cytometry.Apoptosis was measured by Hoechst33342 staining.Results VEGF siRNA stably transfected cell lines were successfully established.The siRNA targeting human VEGF gene effectively decreased the expression of VEGF at RNA and protein levels,and inhibited the proliferation of HeLa cells (P